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This is reminiscent of Cryptococcus neoformans, where discrete foci coalesce into one spot only upon entry into mitosis [ 55].
We first aimed to determine whether the loss of sister chromatid cohesion upon depletion of splicing factors occurred already during interphase or only upon entry into mitosis.
These pro-arrhythmic actions of FPL whether at the cellular or the whole-heart level were dependent not only upon entry of extracellular Ca2+ but also on availability of releasable SR store Ca2+.
Inhibition of pol II (by α-amanitin treatment, RNAi approach, and high dose UV irradiation) has been found to induce p53-dependent apoptosis associated with translocation of p53 to mitochondria, but only upon entry of cells into S phase [ 11, 54] or without entry into S phase, yet in a p53-independent way [ 13].
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While the γ-TuC localises predominantly inside the nucleus throughout the cell cycle (Ding et al., 1997), Pcp1 stays in the cytoplasm during interphase and enters the nucleus only upon mitotic entry.
Sgo1 is absent in G1 and produced only upon cell cycle entry (Marston et al., 2004).
Scc2 levels are unchanged throughout the cell cycle, whereas Scc1 appears only upon cell-cycle entry [ 19, 24].
Although condensin is present in G1 cells and localized to the nucleolus, it begins to co-localize with kinetochores only upon cell cycle entry (Bachellier-Bassi et al., 2008).
The "don't ask, don't tell policy" prevented the military from asking about orientation during the application process, he said, so the only people who were rejected upon entry were those who voluntarily offered that information in an interview.
In contrast, loss of SGOL1, a protein essential only for maintaining centromeric cohesion upon entry into mitosis (Salic et al, 2004; McGuinness et al, 2005), did not alter the distance between sister chromatid signals (Fig 4A).
Labelling of endogenous PKCε by immunofluorescence showed similar localization patterns: wild-type (Fig. 2B) and hly (Fig. 2C) Lm recruited PKCε upon entry (5 min), but at 30 min only vacuoles with wild-type Lm contained PKCε.
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