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Most metabolomic studies a decade ago had a relatively simple experimental design in which the intensities of compounds were compared between only two sample groups.
However, only two sample GEIs for each subject were used in these experiments, which was not sufficient to train a good parameter.
I was able to try only two sample scenes of Peter Jackson's King Kong from Ubisoft, but it's definitely heart-pounding when your realistically hairy ape holds open the jaws of a tyrannosaurus until your paws give out and blood fills the screen.
One of only two sample loops on the whole LP, in fact.
There were three independent RNA samples of pooled ovaries for each growth factor treatment (except for GDNF, which had only two sample replicates), and corresponding control samples for a total of 38 RNA samples.
The MLNE method did not provide any improvement with only two sample time points.
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Rutile and gismondine were identified in only two samples (Table 1).
Blood and urine sampling was performed daily at 24-hour intervals with some patients therefore having only two samples performed.
Results also indicated that only two samples per day, taken morning and evening, could faithfully reproduce the diurnal slope calculated from 3 or 4 samples (r = .97 .99).97 .99
However, contrast groups are only two samples obtained by limited applications or tests on A and B, and may be with missing values.
In our procedure, experiments with only two samples of different mass are necessary to establish if linear isotherms can be used to describe the retention of the contaminants.
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CEO of Professional Science Editing for Scientists @ prosciediting.com