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Exact(17)
Hierarchical clustering analysis of GSE6008 demonstrated that the most positive cluster for the 39-gene signature contained only serous specimens (N = 19, 100%) (Figure 1, far right cluster).
Subsequent work suggested that only serous tumours may be so influenced (Jordan et al, 2007, 2008).
Results were unchanged when only serous tumors were included (Ptrend = 0.54).
Only serous adenoma and serous EOC were included, five benign, five stage IA, and five stage IIIC.
The validation was performed in an independent more homogenous set of 86 EOC patients with only serous histology.
OSPC2, the only serous tumour with mixed clear cell histology evaluated in our series, was also found to highly overexpress c-erbB2 (data not shown).
Similar(43)
The tumour set from this study comprised only 44% serous EOC analysed by immunohistochemistry compared with our report wherein 100% serous EOCs were analysed by microarray, possibly attributing the discrepancy to multiple histological types.
All of the markers and combinations in this analysis had higher sensitivity at 98% specificity when only the serous EOC cases were considered, as compared to all cases of EOC.
Most notably, loss of normal FHIT transcript and impaired expression of Fhit protein occurred only in serous adenocarcinomas of grade 2 and 3 (5/15; 33% and 6/19; 32%, respectively).
Only one serous benign tumour demonstrated moderate staining for PDK1, and it was mostly confined to the inclusion cysts present in the section.
Although the functions of the RAS RAF MEK ERK pathway and its downstream effectors have been recently explored, only the serous type of ovarian cancer has been studied (Hsu et al, 2004; Pohl et al, 2005).
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