A third group received LV-MSK-1, on only one side (Supplementary Material, Fig. S1).
Furthermore, conserved female-biased genes did not show differences in the rate of evolution compared with genes with female-biased gene expression in only one species (supplementary table 4, Supplementary Material online).
Genes with conserved male-biased expression had significantly higher d N /d S values than genes of all other groups, whereas there was typically no significant difference between genes with male-biased expression in only one species (supplementary table 4, Supplementary Material online).
Among these genes, 1804 genes were associated with more than one disease and 784 were associated with only one disease (Supplementary Material available online at http://dx.doi.org/10.1155/2013/637424, Table S2).
As a control, we used a probe containing two copies of the Sall2-binding sequence described by Gu et al. Sall2 binding to a probe containing two recognition sites was significantly stronger compared with a probe containing only one site (Supplementary Figure 5C).
For lymphoma there were two, in 1976 and 1997 (Supplementary Figure S2), whereas for neuroblastoma there was only one, in 1987 (Supplementary Figure S3).
The specificity of antibodies was previously tested by immunoblotting and showed only one specific band (Supplementary Figure 1).
Both genes are tagged by 2 insertions, but for Kuzbanian only one is extracellular (supplementary material Table S1).
We identified 321 core genes AE in muscle tissues (Fig. 3b), but only one in the blood (Supplementary Fig. S7).
The OsHK5, which shows the highest amino acid identity to AHK2, has only one putative TM domain (Supplementary Fig. S2).
Since fecundity rapidly increased (after only one generation; Table 1; supplementary material Fig. S3), this phenomenon may be a result of a biased sampling of the early progeny laid by the most precocious egg-laying females.
