Besides multinucleated macrophages some macrophages with only one nucleus were found and 11(F)).
(The Figure 7A panel illustrates a region of severe PR degeneration in which the ONL is only one nucleus thick).
Furthermore, in an optimal standard cross section (5 10 µm) binucleated cells cannot be detected, because usually only one nucleus is sectioned at a time.
DAPI staining (4',6-diamidino-2-phenylindole stain, [ 19]) showed that the distribution of nuclei in individual cells of RCOL-1 was not uniform (Fig 2), with some cells containing only one nucleus (1.3% out of 1210 cells counted), some with two nuclei (95.9%), and some multinucleate (2.8%) with up to 5 nuclei observed per cell.
The single-cell C4 system is thus unique in that there is only one nucleus for the transcription of genes encoding photosynthetic proteins that accumulate specifically within only one cellular compartment and, most notably, within only one of the two compartmentalized chloroplast types.
If these transcripts code for expansin proteins that play a role in loosening walls in A. acetabulum, it would be interesting to see if their mechanism of action is similar to that in land plants, and whether their substrate is also a cellulose wall. A. acetabulum, for most of its life cycle, contains only one nucleus, which is located in the rhizoid.
While half the ovules in both HAM1/ham1-1; ham1-1/ham1-1 ham1-1/ham1-1 ham1-1/ham1-1liques were mature sHAM2/ham2wild-type siliques shape (Fig. 4G and 4I), the remaining ovules contained only one nucleus localized to the micropylar pole (corresponding to the stage FG1; Fig. 4H and 4J).
It is possible that only one of nuclei of myocyte exhibits TUNEL-positive staining when it undergoes apoptosis at early stage.
To overcome potential difficulties deriving from the syncytium organization of Tuber hyphae, which upon transformation become a mosaic with only one transformed nucleus surrounded by a multitude of untransformed nuclei, we used TAIL-PCR as a sensitive tool to recover flanking-DNA sequences (Liu and Whittier [1995]).
Potential drawbacks are that FCSS cannot detect adventitious embryony and is further not applicable to rare forms of gametophytic development with four-nucleate embryo sacs, where only one polar nucleus is formed (e.g. Carman, 1997).
