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Not only is unbinding unnecessary, it's dangerous.
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The added benefit of using force mapping is that not only are data obtained with respect to unbinding forces, but, simultaneously with imaging, one can also map the stiffness of the cell membrane.
To confirm that the mobility of bound Dystrophin can only be due to exchange with the free-diffusing pool, we tested for lateral mobility of bound huDysGFP, that is, we searched for evidence that huDysGFP can move along the membrane without unbinding and becoming part of the cytoplasmic pool.
Unbinding is also noted but is only complete upon full DNA relaxation (F ≤ 1 pN) for at least 20 min (see Supporting Information).
Indeed, the plot is perhaps Shakespeare's most openly screwy, which is perhaps the best argument for a calculated reserve in staging but is more often perceived as license for the unbinding of directorial giddiness.
His new novel is The Unbinding.
Of immediate importance at the end of M phase is the unbinding of APC and Cdc20 by Cdc14.
I strongly recommend that these descriptions of the steps of unbinding be mentioned only in passing and clearly described as speculation.
The nonlinear response of the system is mediated by unbinding the elongated polymer chains from the particles.
Binding is followed by unbinding events depending on the on- and off- rate of each cross-linker type.
Unbinding is rare, because both subunits need to unbind at the same time for RP to leave the tetramer.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com