This, however, only surfaces in one sequence, a brief action montage with wacky cut-out impressions of distant explosions.
There are 36 (11.1%) variable amino acid positions and of these, 17 variants (47.2%) only occur in one sequence.
There are nine (2.8%) variable amino acid positions and of these, five variants (55.5%) only occur in one sequence.
Although some of the apparent SNPs that were only present in one sequencing read might be due to cloning artefacts, the current results are not surprising in view of the fact that L. perenne is an outcrossing species and the cultivar we used for sequencing is based on a population of several maternal lines and is thus heterogeneous and heterozygous.
EGMM, which assigns multiple foreground classes, has the inverse problem: it gets drowned in false positives, only performing reasonably well in one sequence (MW03, Figure 6).
In the 30 35% sequence identity range, the three methods in the figure appear to have correctly aligned only ~30% of the residues in one sequence-structure pair [PDB codes 1SU4A (sequence), 1XP5A (structure)].
Although by genotyping variants detected in one sequencing pool only we might have discovered additional rare obesity-associated variants, we did not apply this strategy because of lower sequencing overage in some regions and risk of false-positive variants.
Two putative cellulolytic proteins are found in strains ACN and EAN, with a signal peptide predicted only in one EAN sequence.
We thus developed a Perl script to only count the number of redundant nucleotide sites involved in the SNP detection within each contig, thus excluding non redundant nucleotide sites, which were only represented by one sequence in the contig alignment.
Tyr9 is substituted in only one sequence (by Phe), and Pro5 is replaced by Tyr or Phe in two sequences from Mycobacterium species.
