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In AD patients, these aggregates are often found to be tightly associated with neuronal GM1 ganglioside lipids, suggesting an involvement of GM1 not only in aggregate formation but also in neurotoxic events.
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No aggregates were observed in the absence of both pre-differentiation and Y-27632, however either of these alone or in combination resulted in aggregate formation, with the largest aggregates formed in the presence of Y-27632 (Figure 2B).
Thus nitration might have some role in aggregate formation in ALS.
Additionally, we found that temperature changes also played a role in aggregate formation.
This suggests that cellularly secreted components play a role in aggregate formation.
Furthermore, what is the role of proteins carrying ALS-associated mutations in aggregate formation?
Oxidant exposure resulted in aggregate formation, consistent with oxidative protein crosslinking.
One problem in comparing across all screens is the different disease models that have, for example, used toxicity in all or only in specific neurons or alternatively protein aggregate formation in either neurons or muscle as the disease phenotype [ 5, 6, 17].
In addition, aggregate formation might leave less free CTF remaining in the sucrose-soluble cytosolic fraction.
Only GSH supplementation suppressed enhanced aggregate formation in C. elegans bodywall muscle cells.
It is not known whether the process which results in platelet aggregate formation might also activate platelets.
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