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The survival data were fitted by least squares only for experiments with at least three available data points.
Samples with H2O instead of DNA and DNA from uninfected mice brains (only for experiments with borrelia genes) were used as negative controls.
Precautions taken in the manipulation of RNA included separate equipment and materials to be used only for experiments with RNA as well as the use of Diethylpyrocarbonate (Sigma) and RNasin (Promega).
As will become clear below, this is true only for experiments with short RTs.
The obtained model 1 exhibited good prediction capability only for experiments from the 300-mM series, providing reliable information on the final conversion.
Since it has been shown that maximum apoptosis occurs at 3 days after retinal detachment [ 4], TUNEL staining was performed only for experiments 1 and 3. Three retinal sections showing the optic disc were selected randomly for each eye.
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Proportion of tillers with aphids per pot and colony size (number of aphids per tiller) as affected by the endophyte, host genotype, and herbicide dose was analyzed using Poisson distribution and log link function only for experiment 1.
This is not only true for experiments in the wet lab, but also for simulations of computational biology models [ 1].
Sample size was only calculated for experiments performed ex vivo.
Cells were only used for experiments if >90% had a rounded, quiescent morphology.
All sorted cells were analysed by flow cytometry and only used for experiments if the purity was 95 98%.
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