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The most abundant variants were FAKex:15,16, FAKex:14,15,16 and FAKex:13,14,15,16 while the detection of FAKex:13,15,16, required 40 PCR cycles and was only detected as traces.
Instead, BiFC was only detected as a punctual fluorescence within the cells.
As expected the double mutant was only detected as a monomer (Fig. 2C, lane 8).
Interestingly, under non-denaturing conditions, DAPKdel is only detected as a monomer (MW = 33108±1 Da; Fig. 1E to 1H), even under low ionic strength conditions previously shown to promote DAPKwt dimerization.
The majority of genes differentially expressed in RNA-Seq were only detected as present in RNA-Seq, which is important for studies with smaller effect sizes where the sensitivity of hybridization-based techniques could bias interpretation.
The largest subset (591 genes) of the RNA-Seq DE genes were only detected as expressed in the RNA-Seq analyses, so evidence for their DE is also exclusive to the RNA-Seq data.
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Remarkably, we did not only detect as expected the full-length ETV1 transcript but also a fusion transcript (Figure 2A).
Phosphorylation of Mut-3 was only detected at Ser-302, as expected from the frameshift (Fig 2).
For the HMEpC, only CPA4 was detected as N-glycan.
For L2RhZ (see Fig. 3a), Rh3+ was the only detected species as deduced from the binding energy of the Rh 3d5/2 photoelectron peak at 308.9 eV.
Visually the QTL effect was not discernible, and differences were only detected by measurements (as described thereafter).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com