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However, since only a few substrates can hold such an extreme synthesis temperature, the deposition temperature of PCF rarely exceeds 1200 °C.
Although only a few substrates of NEDDylation have been reported in the literature [25], [26], [27], our screen did detect LGALS3, which was previously shown to be NEDDylated using an alternative proteomic approach [27].
Only a few substrates for YopH have been characterized so far, for instance p130Cas and Fyb, but in view of YopH potency and the great number of proteins involved in signalling pathways it is quite likely that more proteins are substrates of this phosphatase.
Only a few substrates of the Brk tyrosine kinase have been identified, the most recent being STAT3.
As only a few substrates of SIK3 are known, the molecular basis of how SIK3 regulates mitosis remains to be defined.
While the roles of SnRK1 members have been reported in various cellular responses, only a few substrates have been identified so far.
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This result indicates that large DegP cages only enclose a few substrate molecules at a time, even in the presence of a large molar excess of substrate.
Although USP11 has been shown to interact with a diverse array of cellular proteins (Bouwmeester et al, 2004; Yamaguchi et al, 2007), its de-ubiquitination activity has only been shown for a few substrates (Ideguchi et al, 2002; Lin et al, 2008).
Despite extensive studies on the functional roles of the mTORC1 signaling pathway, only a few direct substrates of mTORC1, including 4E-binding proteins (4E-BPs), 40S ribosomal protein S6 kinases (S6Ks) (Martin and Blenis, 2002; Hay and Sonenberg, 2004), the autophagy inducer ULK1 (Hosokawa et al., 2009; Kim et al., 2011), and PRAS40 (Oshiro et al., 2007; Wang et al., 2008), have been found.
However, only a few target substrates of RNF2 has been identified up to date and its role in autophagy regulation is still unknown.
However, the substrates that BACE1 processes to regulate these functions are unknown, and to date only a few β-secretase substrates have been identified through candidate-based studies.
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