Randomly selected base substitutions and indels from each MA line were directly examined using Sanger sequencing, and each of 75 base substitutions (supplementary table S2, Supplementary Material online) and 67 indels (supplementary table S3, Supplementary Material online) were validated, supporting the accuracy of the mutation analyses.
The genotype-specific sequence was validated through online BLAST algorithms.
The resultant homology model was validated using online PSVS suite [ 47] which comprises a number of software namely, DSSP [ 48, 49], pdbStat (5.9), AutoAssign [ 50], RPF Analysis [ 51, 52], PDB validation server, Verify3D (1.0) [ 53], ProsaII (2003) [ 54], PROCHECK (3.5.4) [ 55].
The online SPE LC/MS/MS method for waters samples was validated in terms of specificity, linearity, limit of detection, matrix recoveries and inter-day precision of the technique.
The checklist was validated by two physicians and is available as online supplemental material.
Finally, I was validated!
The result was validated by a multiple sequence alignment of 341 protein sequences homolog to GCK and extracted from the non redundant sequence database RefSeq, available online [16].
In one study, only two-thirds of the online responses were validated; in the other study, it was three-fourths.
The online quizzes were validated by the student teacher's supervisor prior to being given to the students to ensure consistency with the required outcomes and provide face-validity.
Subsequently it is refined as a recursive tool permitting an online monitoring; it is validated on laboratory test bench.
