Oligonucleotide primers (supplementary table S1, Supplementary Material online) were designed using Primer Designer software v1.01 (Scientific and Education software).
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Novel qPCR primers (supplementary table S2, Supplementary Material online) were designed to produce small (∼280 bp), efficiently amplified products.
A total of 138 pairs of primers (supplementary table S5, Supplementary Material online) were designed to cover the whole plastid genome (supplementary fig. S1, Supplementary Material online).
The software, open-source and freely available online, is designed for learning disabled children aged 5 8, and may also be useful for general instruction of normal preschool children.
The qRT-PCR primers (supplementary table S1, Supplementary Material online) were designed with Primer Premier 5.0 software (Premier Biosoft International, Palo Alto, CA).
Outbound primers (forward and reverse; supplementary table S2, Supplementary Material online) were designed from the coding region of each minichromosome; the two primers in each pair were next to each other with a small gap or no gap in between.
Primers for standard PCRs (supplementary material S1, Supplementary Material online) were designed with Primer3 (Rozen and Skaletsky 2000) on the two complete M. senhousia F- and M-mtDNAs (GenBank accession nos. GU001953 4).
