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(ii) BLAST search: Users can use an online BLAST interface to input an interested sequence in FASTA format and search against all nucleotide or protein sequences in our database (Supplementary Figure S1B).
The 16S rDNA sequence was compared with other bacteria using online BLAST tool with deposited sequences of Genbank database.
We used primers (Thermo Fisher Scientific, Waltham, MA USA) designed in-house using primer3 and mfold software, as well as the NCBI online Blast tool (https://blast.ncbi.nlm.nih.gov/Blast.cgi), sequence available on request [10, 21, 22].
Subsequent sequence manipulations utilized the online BLAST 2 Sequences [37] (http://www.ncbi.nlm.nih.gov/ blast/bl2seq/bl2.html).
The method has advantages compared to the online BLAST search tool, by including phylogenetic information and providing statistically meaningful measures of confidence (posterior probabilities) to the taxonomic assignment.
In-silico analysis using the online BLAST program at NCBI indicated that the M. smithii and M. stadtmanae real-time PCR systems were specific for the two species.
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He already removed Bernier from his shadow cabinet in June after the latter posted a book chapter online blasting the CPC leaders as having won his post illegitimately.
Bynes — who was well-known for her online blasts a couple of years back as she managed her mental health in public — tweeted only four times in 2015 and once previously this year, to show off a new haircut, so seeing her nine tweets in a row Wednesday had a hint of the Old Amanda.
Comparison of the determined sequence with those in the GenBank/EMBL database was made using the online tool BLAST programme [29].
The 16S rDNA sequence was analyzed and compared with other deposited sequences in the Genbank via the online programme BLAST (http://www.ncbi.nlm.nih.gov/).nih.gov/
Similarity comparison of each DNA sequence recovered from the DGGE gel was performed using an online program (BLAST, http://www.ncbi.nlm.gov/BLAST).nlm.gov/BLAST
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