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Both cysteine domains were aligned using the European Bioinformatics Institute ClustalW online alignment tool (http://www.ebi.ac.uk/Tools/msa/clustalw2/), and the TEXshade alignment shading software (Beitz, 2000) was used to create the graph.
The amplified partial 18S rRNA gene was sequenced and compared with similar information available at the GenBank by an online alignment search.
It converts output from the GenBank "Blast 2 Sequences" online alignment software (www.ncbi.nlm.nih.gov) to a manageable database of identified sequence variants.
Online data analysis has not been addressed; segmentation of threads and online alignment of outputs seem difficult to achieve owing to the sharing restrictions imposed by GPGPU.
The forth, Alu locus 143 contains all five of the AluYa5 diagnostic nucleotide substitutions (supplementary file S5, Supplementary Material online, alignment file).
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Furthermore, considering the average genomic noncoding divergence (see supplementary table S2, Supplementary Material online), alignments of our whole coding threshold divergence levels are less likely to be observed by chance (binomial P < 0.05) in all lineages.
For online sequence alignment, use the "align 2 sequences" BLAST tool at NCBI (www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi).nih.gov/blast/bl2seq/wblast2.cgi
The newly obtained sequences were deposited in the European Molecular Biology Laboratory nucleotide sequence database (accession numbers FR669194-FR669243 and FR675968-FR675974), aligned using the online Silva alignment tool (Pruesse et al. 2007), and merged with the ARB database using ARB software package version 5.1 (Ludwig et al. 2004).
An online sequence alignment program, mVISTA LAGAN [ 78] was used for global pair-wise sequence alignment [ 15].
An integrated pipeline for online sequence alignment and phylogenetic tree construction is also provided.
The CBCT has soft tissue resolution, which allows direct online target alignment prior to each treatment session.
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