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The majority of single-base homoplasmic variants (88.48%) was already described in healthy individuals [ 18] and 62.75% heteroplasmic ones were annotated in public databases in a homoplasmic state (Additional File 9).
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The remaining missing ones are annotated as putative transposases or IS elements that have not been deposited in ISfinder (column IPuative and INovel of Additional file 1, Table S2).
Each one is annotated by the network's uncannily informed host, Robert Osborne; his introductions and afterwords, peppered with facts -- on-the-set anecdotes, placement within the career of a performer or filmmaker -- find distinction in even the least inspired studio release.
Only two genes were in common across all three datasets and only one was annotated.
Five of them are coding variants, of which one is non-synonymous and one is annotated to be in a splice site of Gtf2i.
This gene is the same as the one being annotated encoding a putative L-arginine amidinotransferase (see below for discussion of this aspect).
One is annotated as H0UX35_CAVPO and shares 98.8% identity with human threonine aspartase (all sequence alignments performed with Clustal Omega); a second is annotated as H0W0T5_CAVPO and has 70.4% identity with human 60 kDa lysophospholipase.
Of the 10 genes that had significantly low (or absent) transcriptional activity in the Florida strain (Table 4), only one is annotated with a predicted localization: gene AMF_878, coding for outer membrane protein 4 (OMP4).
All these classes but one are annotated with a GO Biological Process term related to 'Signal Transduction', or are nested within a larger class annotated with such a term (by nature, a nested class inherits the annotations of all its parent classes) (Additional file 2).
An example of one such model is triglyceride module five from the MMC analysis that is also strongly correlated with weight and sugar and contains only three genes, of which only one is annotated to have any putative function (cytochrome P450).
Among these peptides, 113 were contaminant ones, 12 619 were annotated ones and 103 were novel ones.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com