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In contrast to their strong impact on the steady state levels of a variety of pervasive transcripts, including previously unannotated ones, inactivation of nuclear and/or cytoplasmic RNA quality-control pathways had a relatively minor global effect on the steady state levels of coding transcripts.
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For NEJM subscribers who may have missed the original article last October in Science, Scripps Research Institute's Dr. Daniel R. Salomon describes how researchers at Harvard "genetically engineered a one-step inactivation of more than 60 copies of PERV, thereby reducing the infectious risk from PERVs by three orders of magnitude".
In one example, inactivation of chrysotile by sodium alginate, depolymerization by ascorbic acid was also ineffective.
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
More recently, the phage λ red recombinase has been successfully applied to allow rapid, one step inactivation of genes (4).
The MPS14 strain was constructed by the one-step inactivation of E. coli chromosomal genes described by Datsenko and Wanner [ 39].
Only one somatic inactivation of RB1 is required in hereditary patients to develop retinoblastoma and therefore they are often affected bilateral.
The E. coli K12 BW25113 Δ acrAB and E. coli NST74 Δ acrAB strains were constructed using the one-step inactivation of chromosomal genes using published methods (Datsenko et al., 2000).
Clonality in EPCs from MM patients was determined by X-chromosome inactivation (XCI) patterns using a highly polymorphic CAG repeat in the androgen receptor (AR) gene, a well-established method to assess clonality of tumor cells [ 14] based on random epigenetic inactivation of one of the two X chromosomes inherited from either parent [ 15].
An Arrhenius-type model, consisting of one term for the activation and one for the inactivation of the microbial catalyst, was found to fit the whole specific growth rate versus temperature curve.
A paradoxical activation of pAKT was observed in one patient with inactivation of pHER2 and pERK1.
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