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We measured AGT and renin expression and ANG II concentration in cardiac myocytes isolated from AT1a-KO mice after one wk of diabetes.
We previously reported that aliskiren was better than benazeprilat and candesartan in reducing cardiac ANG II levels and preventing diabetes-induced cardiac myocyte apoptosis, oxidative stress, and cardiac fibrosis after one wk of diabetes in rats [ 8].
We had previously demonstrated intracellular ANG II synthesis in neonatal and adult cardiac myocytes following stimulation with high glucose in vitro and in rat hearts after one wk of diabetes [ 8, 9].
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Western analysis of β-actin integrity demonstrated that one wk ambient storage of testicular tissue in RNA later did not affect actin integrity as evidenced by the absence of proteolytic fragments and consistent signal intensity in replicate samples).
In addition to the 10 wks study, a short study of one wk was performed to measure cardiac RAS activation.
The anti-inflammatory effects of cysteamine lasted for one wk beyond its removal from the experimental system.
Ovaries stabilized in RNA later for one wk at RT and two wks at 4°C were used for protein extraction.
One wk later draining popliteal LN cells were harvested, labeled with CFSE, and restimulated with titrating doses of KLH in vitro for 3 d.
Prenatal diet was measured at only one time point during pregnancy, 32 wk of gestation; therefore, we do not know whether the results are different for early pregnancy.
In early pregnancy the uterine-artery Doppler waveform is characterized by a high-resistance profile, transforming to one of low resistance by 24 wk of gestation in normal pregnancies.
There were 297 women who underwent at least one Doppler study between 18 and 32 wk of gestation.
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