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Cells were incubated at 37°C for 60 minutes at a density of 4 × 105 cells/well in serum-free DMEM on non-coated well plates (three wells/cell line: one well to control the number of seeded cells and the two others for technical duplicates).
On a 384 microtiter plate the spacing from the center of one well to another is 4.5 mm (exactly double the spacing in a 1536 well plate).
On the day of viral supernatant harvesting, count the number of cells in one well to determine cell number at time of infection.
That leaves only one well to serve the city.
(b) 1 ml PBS is added to one well to humidify the surrounding.
Like a juggler tossing two balls between his hands, Petta can move the electrons from one well to the other by selectively toggling the gate voltages.
Similar(35)
Few movies of recent years, for example, have been more violent or have seemed more violent, for little overt brutality is shown than the two versions of "Funny Games," in which a pair of white devils in tennis gear invade and torment one well-to-do family after another, on the shores of a quiet lake.
Michel Blanc's "See How They Run," based on Joseph Connolly's novel "Summer Things," is a contemporary bedroom farce about two families, one well-to-do, the other in dire financial straits but too ashamed to admit it, who vacation in the same seaside town.
One well-to-do young lady has taken this love further and remade MC Jammer's "Murkle Man" video.
In the end, society is left with one well-to-do group unable to recognize truth outside scholastic walls, while the other group extols revivalism at the cost of the intellect.
This is to miss an important point, one well known to public health experts but strangely ignored by most politicians.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com