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After incubation, the mixtures were placed on ice, and one volume of 2× Tris-EDTA buffer was added.
One volume of the trilogy, "Mirabell: Books of Number," received the National Book Award in 1979.
3. Jorge Guillermo Borges, father of Jorge Luis Borges, also published one volume of fiction with a private press.
One volume of Tolstoy's fictionalised history of Russia during the Napoleonic campaign will be read each day.
2. Seepersad Naipaul, the father of VS Naipaul and Shiva Naipaul, published, with a private press, one volume of fiction.
Five volumes of buffer and one volume of 111InCl3 (Covidien, Petten, The Netherlands) were added.
The cell biomass was washed thrice with one volume of sterile distilled water.
One volume of 20% trichloroacetic acid (TCA) was added.
One volume of a 20% NaCl solution and one volume of a detergent solution (20% sarkosyl, 2% SB3-14 and Tris 20 mM pH 7.4) were added to one volume of 20% brain homogenate.
One volume of 2% SDS was added and the OD600 measured.
One volume of isopropanol was then incubated at −80°C supplemented with 500 µl from the supernatant.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com