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Eighty-nine percent (41out of 46) of the higher grade culture positive samples (3+ and 2+) were positive by DLRPA at more than one time points of RLU readings between day 7 and day 21 while 60% (15 out of 25) of the lower grade (1+, few-colony-growth) and culture negative samples were positive only at the end of day 21.
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However, a majority of the abovementioned microarray studies on cell cultures use only one time point of RNA analysis after stimulation.
miRNAs whose expression varies in at least one time point of elicitor treatment are listed (for details on the entire set of miRNAs represented in the microarray, see Additional file 1: Table S1. The fold change (elicitor-treated vs non-treated plants) for each miRNA is shown.
Given the remaining pre-analytical technical challenges in preparing a large number of samples for epigenetic analysis, we assessed only one time point of ALI-induced sepsis and have focused on Angpt1, Tek, and Kdr, which exhibited the most pronounced mRNA downregulation.
Nevertheless, one time point of 48 h is a good compromise to obtain a quantitative value that can be correlated with other variables such as the tumor response.
Nevertheless, one time point of 1 h is a good compromise to obtain a quantitative value that can be correlated with other variables.
We also only examined one time point of morphine-induced cardioprotection with morphine given prior to ischemia.
This number was noticeably smaller than the number of genes expressed during the development of cotton fiber [ 10], but we only sampled one time point of petal development.
31 Fourth, we had only one time point of maternal BG level measurement, which may not represent the true level of maternal BG.
Thus, this limitation of our data would strengthen the conclusion that one time point of remission in no guarantee for sustained remission.
Genes identified as differentially expressed in at least one time point of the global transcriptome analysis performed to study cellulase induction in NG 14 and RUT C30 strains.
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