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However, a majority of the abovementioned microarray studies on cell cultures use only one time point of RNA analysis after stimulation.
miRNAs whose expression varies in at least one time point of elicitor treatment are listed (for details on the entire set of miRNAs represented in the microarray, see Additional file 1: Table S1. The fold change (elicitor-treated vs non-treated plants) for each miRNA is shown.
Given the remaining pre-analytical technical challenges in preparing a large number of samples for epigenetic analysis, we assessed only one time point of ALI-induced sepsis and have focused on Angpt1, Tek, and Kdr, which exhibited the most pronounced mRNA downregulation.
Nevertheless, one time point of 48 h is a good compromise to obtain a quantitative value that can be correlated with other variables such as the tumor response.
Nevertheless, one time point of 1 h is a good compromise to obtain a quantitative value that can be correlated with other variables.
We also only examined one time point of morphine-induced cardioprotection with morphine given prior to ischemia.
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Eighty-nine percent (41out of 46) of the higher grade culture positive samples (3+ and 2+) were positive by DLRPA at more than one time points of RLU readings between day 7 and day 21 while 60% (15 out of 25) of the lower grade (1+, few-colony-growth) and culture negative samples were positive only at the end of day 21.
Longitudinal A/D data were available for more than one time point for 94% of the genotyped children.
For studies reporting more than one time-point of documented clearance, the latest time-point was included in the analysis.
In summary, during isoniazid therapy, we observed a significant change of the IFN-γ response to CFP-10-1, Rv0849, Rv1986, Rv2659c Rv2693c, Rv2693c and Rv1737 for at least one time-point of the study.
Another limitation of our study is that the birds were sampled only at one time-point of the APR, which means that we could have missed other differentially expressed transcripts that appear before and after the 12 h time-point of sampling.
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