At six weeks of age, the mice were gavaged with 200 μl of DMBA (1 mg/ml in sesame oil) one time per week for six weeks [ 42, 43].
The statistical significance for a linear trend was tested by assigning a value to each exposure category (0 for nonusers, 0.5 for less than one time per week, two for one to three times per week, five for four to six times per week, and seven for daily users) and including it as a continuous variable in the logistic regression model.
There were five alternatives: (1) Never; (2) Irregularly; (3) One time per week; (4) Two times per week; and (5) Three or more times per week [ 24].
Training consisted of four consecutive 10-12 min sessions per day, administered two times per week for two months, which then tapered to one time per week for two additional months.
Compared with nonusers of multivitamin supplements, the multivariate-adjusted ORs after controlling for traditional diabetes risk factors were 1.03 (95% CI 0.91 1.17) for less than one time per week, 0.93 (0.85–1.01) for one to three times per week, 0.85 (0.78–0.92) for four to six times per week, and 0.96 (0.92–0.99) for seven or more times per week (P for trend = 0.008).
We need to follow the example of the Netherlands which just this year recommended reducing fish consumption from two times per week down to only one time per week.
Both questions asked about any use in the past 12 months and asked users to mark how frequently they took them: less than two times per month, two to three times per month, one to two times per week, three to four times per week, five to six times per week, one time per day, or two or more times per day.
The mothers chose one of the following responses: "none", "one to two times per week", "three to four times per week", "five to six times per week", "one time per day", "twice per day", "three times per day", or "four or more times per day".
Nocturnal awakenings occurred one time per night for at least two nights per week accompanied with difficulty in resuming sleep (awakening duration ≥ 30minutes).
Participants were also asked about their average frequency of physical activity (never, one to two times per week, three to four times per week, five times and above per week) and average duration of physical activity (less than half an hour, half an hour to 1, 1 2, more than 2 h).
The LiFE programme activities, which could be performed daily, were recoded to correspond with the other two programmes (up to one day of activities performed in the LiFE programme=0 times per week of exercises performed in other two programmes, two to three days=one time per week, four to five days=two times per week, and six to seven days=three times per week).
