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Using a classical one-factor-at-one-time method followed by a response surface methodology, culture conditions were optimized to obtain simultaneously high yields of biomass (1.10 g/L) and high reduction activity (39.49 μmol/min/g with the model substrate 4'-chloroacetophenone).
Based on one at a time method, the optimum flow rates of H2, air and N2 were 375, 259 and 2.6 mL min-1, respectively.
For politicians, there is one time-tested method for thwarting public records requests: stall and stall and, when in doubt, stall.
Effect of initial sample volume and type, volume and concentration of eluent on the ER%Cr III) were investigated and optimized using one at a time method.
The pH was optimized by one at a time method and fixed at 5.0.
All experimental parameters affecting the AgNP synthesis and CMC determination were optimized by "one at a time" method.
The optimum condition, concluded from one at a time method was 2 mL of 0.1 mol L−1 HCl as extraction solvent and 5.0 min from desorption time.
The most influential parameters for succinic acid production obtained through one at a time method were sucrose, tryptone, magnesium carbonate, inoculum size and incubation period.
The best pH in quaternary system was studied by using one at a time method to achieved maximum dyes removal percentage.
Traditional methods use one at a time method of approach that is laborious and time-consuming and it does not reflect interactions between different variables [ 11].
The use of the full 25 factorial design instead of "one-variable-a time" method allowed to increase the sensitivity of HRP-C determination 2355 fold without a change of detection limit.
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