Sentence examples for one stringency from inspiring English sources

5hmC content on autosomal chromosomes showed significant decreases from one stringency to the next.

One stringency wash (DakoCytomation) was performed at 42°C for 30 minutes.

For nearly all assay strategies, a decrease in success rate was observed in the direction from methods A and B for assigning genotypes to methods C and D. This was especially clear for assay strategies with limited flexibility, e.g. one probe length or Tm-matched probe-set at one stringency, strategy 8 and 9, respectively.

To create the core dataset, we used intervals supported by at least one low stringency (25% FDR) and one high stringency (1% FDR) dataset from independent experimental techniques.

We identified intervals supported by at least one low stringency (25% FDR) and one high stringency (1% FDR) dataset from the independent experimental techniques; for example, all regions confirmed with 1% FDR DamID and 25% FDR ChIP were included, and vice versa.

Washing was conducted in PBS/tween-20 with one high stringency wash at 57°C.

Each round of PCR contained three annealing stages: stage 1, five high-stringency (65 °C) cycles; stage 2, one low-stringency (25 °C) cycle in primary PCR or one reduced-stringency (50 °C) cycle in secondary/tertiary PCR; and stage 3, twenty-five high-stringency (65 °cyclesles.

On the one hand the stringency of this approach ensures high specificity; on the other, misclassification error will tend to accumulate reducing the sensitivity of the overall DSM-IV algorithm.

For each PCR, target single-stranded DNA primed by the SWPOP primer in the exclusive one low-stringency cycle is converted into double-stranded form in the following high-stringency cycle due to the presence of a perfect annealing site for the specific primer.

The one low-stringency cycle (LSC) of primary PCR allowed SWPOP-P to anneal to the target DNA and extend towards SP-P.

The one reduced-stringency cycle (RSC) of secondary/tertiary PCR allowed SWPOP-S/SWPOP-T to bind to the prior SWPOP complementary site(s).