Exact(4)
This point out an additional effect of T4 that also inhibiting PC biosynthesis from ethanolamine through an action on one step downstream P-Etn, i.e. PfPMT, CTP-phosphocholine cytidylyltransferase (PfCCT) or PfCEPT.
Of these, the larger accumulation (10.5-fold) was of dihydroorotate, a pyrimidine synthesis intermediate located one step downstream of the carbamoyl phosphate-dependent step in the pathway.
Fringe acts one step downstream of O-fucosylation, adding N-glycans onto fucosylated sites.
This idea is further supported if the consequences of Dp71 deficiency are studied one step downstream of the channel alterations, by assessing the swelling properties of Müller cells.
Similar(56)
After removing the contaminant oligopeptides using ultrafiltration, purified PAC with a specific activity of 16.3 U/mg was obtained and the overall purification factor for this one-step downstream purification process was up to 3 fold.
Using curated findings from the literature, the shortest path for about one sixth of the 387 nephritis genes was defined to be either 0 or one-step downstream of the rapalog-mTOR pathway.
Figure 4 represents one step further downstream the initial cloning and describes the transfer of cloned ORFs from the entry vector to the destination vector of choice.
With the spare style of a war reporter (his previous book, "Fools Rush In," recounted his harrowing experiences in Sarajevo during the height of the Bosnian war), Carter introduces a man in his early 40s named Boobuck who waded into the river, drunk, to check his subsistence net; he took one step too many and was sucked downstream to his death.
These treatments block autophagic degradation at a step downstream of autophagosome formation, allowing one to make a direct comparison of autophagosome numbers or lipidated Atg8/LC3 levels with experiments done without using these drugs.
For pacC replacement, pUC57 plasmid containing the upstream fragment was ligated in one step using T4 DNA ligase (NEB) to the downstream flank and Hph cassette fragment (Additional file 11).
One step in this direction is thus to understand exactly which downstream genes and cellular processes become affected in the outcome of driver alterations.
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