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The difference was not likely caused by inclusion bodies disappearing because of their small size and the distance between two sections, as muscle fibers negative for Congo Red were positive for p-FTAA, both on the section preceding and the section following the one stained with Congo Red.
Four sections were made at each level, one stained with haematoxyllin and eosin (H&E), one stained with a broad-spectrum anticytokeratin monoclonal antibody (MNF 116) using immunohistochemistry (IHC) and one negative control for IHC with one spare.
The tissue blocks were sectioned to obtain two adjacent sections, one stained with H&E and the other with Feulgen dye.
And one stained with blood.
Similar(56)
Every 4-sections was a group: the first ones stained with cresyl violet for neuronal counting, and the others for immunohistochemistry using specific antibody against WOX1, p-WOX1, c-Jun, or indicated antibodies.
Whole tissue sections from 148 surgical resection specimens (SRS) and 99 matched preoperative core biopsies (CB) with invasive BC of no special type were analyzed on one slide stained with pan-cytokeratin.
All primary tumours except one stained more weakly with anti-UGT8 antibodies than did the lung metastases.
The number of nuclei seen within one frame for one section stained with HE system was counted using an image analysis system.
Ten slides were obtained with one stained by H&E method.
20 μg of recombinant S100A4 were run on two identical 2D-PAGEs of which one was stained with coomassie-blue (Fermentas, Burlington, ON) to use as template for cutting out spots of interest from the untreated gel.
Therefore, there had to be at least one staining with clear clustering from a given replicate to pass the filter.
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