An EMAGE submission generally will be one specimen stained for the expression of one gene or protein at one point in embryonic development.
One slide stained for cytokeratin and one negative control slide were analyzed per patient.
Only three previous reports analyzed the role of the cytoskeleton during frustule formation using fluorescence microscopy [25] [27], and only the most recent one also stained for silica to allow observation of the cytoskeleton and valve structure [27].
Surprisingly, there have been only three previous reports visualizing the cytoskeleton in diatoms during frustule formation using fluorescence microscopy, and only the most recent one also stained for silica to allow correlation between the cytoskeleton and valve structure.
Fifty-nine RMS but only one ES stained for desmin (P less than 0.05).
The embryos were divided into two halves along the midline of the anterior posterior axis, with one half stained for cartilage and bone using Alcian blue and Alizarin red as detailed in Hogan et al. [26].
For FACS analysis, after exposure to different plasmas, TEC were detached from tissue culture plates with EDTA, washed twice with one times PBS and stained for one hour at 4°C with FITC-conjugated antibodies directed to human Fas, CD40, inter-cellular adhesion molecule-1 (ICAM-1) (Becton Dickinson, Franklin Lakes, NJ, USA) or with an irrelevant control antibody.
All analyzed specimens, except for one ductal breast cancer and one normal breast, stained for VDR.
One section was stained for H&E followed by immunohistochemistry for p63/AMACR on the consecutive section.
One section was stained for EGFP and either SMαA, vWF, or eNOS and examined for donor cell incorporation into arterial endothelium.
