Heart dysfunction was induced by two intra-amniotic injections of homologous isoproterenol (ISO) (Sigma, St . Louis MO, USA) with laparotomy on E14.5 and E15.5 or one single injection with laparotomy on E14.5.
Cells were never injected in one single injection, but were rather introduced using multiple low-pressure injections (typically 6 10) at the same site, thereby generating progressively larger cell clusters.
With different schedules – one single injection of 15 mg m−2 mitomycin on day 1 (Kretzschmar et al, 2000) or by combining infusional 5-FU with folinic acid with 10 mg m−2 MMC every 3, respectively every 6, weeks (Hofheinz et al, 2002; Tebbutt et al, 2002) – survival in gastric cancer patients ranging from 5.3 to 10.2 months has been reported.
Administration of one single injection of long-acting progesterone is a simple and effective method of controlling the first ovulation of the season in late transitional mares.
There is still ongoing discussion, how much anti-VEGF drug is required to treat ROP sufficiently with one single injection.
We have observed that one single injection of tamoxifen results in the ablation of PTEN in many epithelial cell types.
For the intravitreal use a monthly dose of 500 μg of ranibizumab (one single injection) was shown to be safe.
We found that intramuscular and oral GCs are equally effective as bridging therapy, but one single injection might be more feasible.
In conclusion, one single injection of ranibizumab was effective in treating ROP 3 plus disease in zone 2 in all cases without showing late recurrence of ROP.
SRM assay technology allowed us to quantify all 51 MPM candidate biomarkers in parallel in one single injection per sample (Additional file 9: Table S6).
In a first experiment, day -1 was chosen for treatment with MSCs because experiments previously performed in our laboratory demonstrated that one single injection of CD4+CD25+ regulatory T (Treg) cells at day -1 significantly inhibited CIA [ 37].
