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Of several inbred strains tested, DBA/1 mice, the same inbred strain of mice that is also susceptible to CIA, develop severe symmetrical peripheral polyarthritis arthritis following one single immunization with recombinant human or murine G6PI in adjuvant [ 93] (Fig. 1).
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Only one single dominant epitope emerges from the immunization of H−2k mice with the whole protein of moth cytochrome c (MCC) or closely related pigeon cytochrome c (PCC) (Hedrick et al., 1982; Winoto et al., 1986; Hedrick et al., 1988; McHeyzer-Williams and Davis, 1995).
Furthermore, protective immunity is induced ideally after a single immunization.
A single immunization resulted in robust cellular and humoral immune response.
Thus, ideally, protective immunity is induced after immunization with lower doses and preferable after a single immunization, which are key elements in the development of pandemic influenza vaccines.
One single person.
Not one single question.
In one single night.
"One single proof".
In the best-case scenario, a vaccine is cheap, easy to administer and offers lifelong protection after a single immunization.
Not one single thing!" .
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