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Chips were from one single batch.
Aliquots of the blood samples were stored at -20°C for the duration of the experiment and were then sent in one single batch for each hormone to the laboratories responsible for the analyses.
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Starting with smaller batches means students can produce multiple lots over the course of the semester, rather than one single large batch as they did in the past.
This can be extended to up to one million in a single batch.
Assays for CRP and TNF-α levels were performed in a single batch by one of the authors who was blinded to the clinical details of individual patients.
To minimize technical variability, all targets were synthesized in one round and hybridization experiments were conducted on slides from a single batch.
To minimize technical variability, all targets were synthesized in one round and each hybridization experiment was conducted simultaneously on slides from a single batch where possible.
To avoid confounding by extraneous factors, all experiments were performed in a single batch and processed by one researcher on the same day for each step.
To avoid confounding by extraneous factors, all the experiments were performed with a single batch and processed by one technician on the same day for each step.
For this reason, all biopsies were obtained in a standardized fashion (wedge biopsies), and one single pathologist rescored all biopsies in batch, blinded for any clinical information.
One hundred and fifty final year medical students from a single batch who were doing clinical appointments in the final year at the CSTH were also invited.
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