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Moreover, conserved regions with ICS are difficult to align well by one single alignment.
All the four genes were merged into one single alignment.
A set of fragments is called consistent if all fragments can be included into one single alignment, see [ 15].
Once the selected MSA programs output one single alignment, where residues are kept in their input order, it is not possible to benchmark their performance.
Then we masked all third codon positions of mitochondrial coding sequences [VIII] and concatenated all tRNA alignments to one single alignment.
Here, consistency means that a set of fragment alignments can be included into one single alignment without contradictions, see for example [ 5] for a more formal definition of our notion of consistency.
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Every possible fragment is given a score based on the probability of its random occurrence [ 20], and the algorithm tries to find a consistent collection of fragments with maximal total score; consistency here means the combinatorial requirement that the selected fragments are required to fit into one single output alignment and do not 'cross' each other.
Multiple alignments of protein sequences were constructed using the M-Coffee web server (http://tcf_dev.vital-it.ch/apps/tcoffee/play?name=mcoffee), which combines the output of several popular aligners into one single multiple sequence alignments.
It is possible that the selected anchor points are inconsistent with each other in the sense that they cannot be included in one single multiple output alignment, see [ 16] for our concept of consistency.
However, it was sufficient to enforce the correct alignment of one single column using corresponding anchor points to obtain a meaningful alignment of the entire sequences where not only the one anchored column but most of the three core blocks are correctly aligned.
If a gene happened to be used in more than one source tree dataset, we superimposed the alignments on the different taxon sets to produce a single alignment on the union of these source tree taxon datasets.
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