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Third, our survey is a one-shot analysis with a focus on only one geographical area and discipline.
It should be noticed that double or triple infection of the same cell is not a problem for the xenoarray approach, because in the one-shot analysis the selection drivers will be present in all selected cells, while the bystanders will be diluted out.
One-shot preliminary analysis can be done with as little as 5 8 μL of sample that is processed either by direct trypsin digest or liquid-liquid extraction before mass spectrometric analysis.
Close statistical analysis has convincingly documented that making one shot -- or five or ten -- does not boost the odds of making the next one.
One shot, one opportunity.
One shot was fatal.
No one shot back.
It took one shot.
The mean, median, and modal number of shots per scene are 11 shots, six shots, and one shot, respectively.
One shot was taken from each location.
When one shot ends and another begins, it is called a "shot change".
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