On day 19, one pup was missing and was presumed to have been eaten by its mother.
Don't work on any tricks, but stare into the soulful eyes of one pup and spend the rest of the hour filling out adoption paperwork.
Neonates from each of five litters (one pup per litter) per exposure group were randomly selected and euthanized on PND1, PND14, PND21, PND45, and PND90.
Each week she tested one pup, so that no pup got used to it.
These statistical modeling analyses raise significant concern about the selection of one pup per litter for highly variable end points.
At those sites, females usually give birth to one pup a year, an added challenge for dropping populations.
When one pup was randomly selected, a substantial percentage of incorrect conclusions about the presence or absence of treatment effects occurred.
Sequence analysis at the targeted Tyr locus revealed indel mutations in three pups (23.1%), and the precise SNP exchange in one pup (7.7%) (Fig. 3c; Table 3).
Of the 13 pups delivered, one pup (7.7%) showed a recovery of the coat-colour for albino with non-agouti, hooded phenotype (C, a, h) (Fig. 3b).
"Devil rays grow very slowly and produce just one pup about every two years, making them intrinsically susceptible to overfishing," said Sebastián Pardo, at Simon Fraser University in Canada.
However, DNA from one pup produced a positive PCR band (405 bp) amplified by a primer set designed for the outer sides adjacent to the two gRNA-targeting sequences (Fig. 3f).
