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To account for our variable sampling effort among orchid species, rarefaction curves were simulated 5,000 times using analytic rarefaction 1.3 [ 84] on two datasets: one pooling all populations for each species, and the other separating each population and calculating a mean value for each species.
However, most of these methods are based on substantial post-PCR processing, and for one or very few SNPs for one pooling sample as a time.
To some extent, the investigator's success in overcoming one pooling problem (e.g., demonstrating that her company can be trusted) is dependent on her ability to overcome the other (identifying wrongdoers amidst a large employee population).
After PCR amplification of the selected fragment, one pooling library was sequenced using Illumina HiSeq™ 2000.
To provide measures of the consistency of differences between pooling approaches under repeated sampling, we plotted the prediction accuracies observed for one pooling approach against those observed for the other in the same 50 data sets.
Four de novo assemblies were performed using CLC Genomics Workbench 5.1: three separate assemblies containing the raw reads of each treatment, and another one pooling all raw reads (called "reference").
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Beginning on Saturday, though, all eyes will be on just one pool in Sydney: the Olympic pool at Homebush Bay.
Ms. Herr threw a minnow trap into one pool on Tuesday to see what she could find.
Thus each set of 30 pathogen-inoculated plants yielded two pooled samples, one pooled from 30 sections from the lower infection courts and one pooled from 30 sections from the upper infection courts.
In the initialization phase, we need one pool of initial solutions and based on our experience, construction heuristic works better that random approach.
On average, we found larvae of three males within one pool at a given sampling session (iqr = 1.75 4).
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