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Based on the alignment due to matching interactions and on the annotation of one of the alignment partners, we predict the function of several ORFs.
At the areas stretching to the borders of the compartment, we use a variant of the local alignment algorithm in which one of the alignment's ends is fixed at the pivot.
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Figure 4 shows the analysis of one of the alignments simulated with five taxa and HGT.
Using the hmmbuild algorithm of the HMMER program (European Bioinformatics Institute), each one of the alignments downloaded from the Pfam database was used in the construction of hidden Markov models.
The probability of false-positive associations is higher in case of rare alleles and cases where a polymorphic site was only detected with one of the alignments.
For this, a non-consensus amino acid has to be present above a user-specified threshold proportion in one of the alignments.
If at least one of the alignments (S r against S oA or S r against S oB ) leads to a hit, the breakpoint sequence can be refined.
Also, the option "-k1" was elected, i.e. if in the course of the search Bowtie found 2 (or more) possible alignments for a given read, the program selected one of the alignments at random.
We selected the F-score because of its dependence on recall and precision and because of the symmetry in the F-score formula, which eliminate the need to select one of the alignments as a gold standard.
As long as the sum of the consensus threshold and the specificity threshold is lower than one, a position can be in the consensus and specific to one of the alignments at the same time (Supplementary Material at http://www.mrc-lmb.cam.ac.uk/genomes/spial/help.html).ac.uk/genomes/spial/help.html
The poly(A) filter (10) helps avoid false positives when one of the alignments associated with the candidate is either a run of T's at the very beginning of the contig or a run of A's at the very end of the contig.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com