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In an experiment named "4 + 4", 0.15 μg of each one of segments 1, 2, 3, and 5 as well as 0.2 μg of either B1 or Env-expressing plasmids were transfected into one batch of cells, and identical amounts of segments 4, 6, 7, and 8 as well as 0.2 μg of either B1 or Env-expressing plasmids were transfected into another batch of cells.
In another experiment named "7 + 1", 0.086 μg of each one of segments 1, 3, 4, 5, 6, 7, and 8 as well as 0.2 μg of either B1 or Env-expressing plasmids were transfected into one batch of cells, and 0.086 μg of segment 2 as well as 0.714 μg of either B1 or Env-expressing plasmids were transfected into another batch of cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com