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The assays included two internal positive and one negative control specimens on each ELISA plate.
Samples were arranged on 25 96-well plates containing one negative control and at least one study sample in duplicate.
Reduced DNA samples were sequenced on the Illumina HiSequation 2000, with 95 samples plus one negative control per lane.
One negative control dog and 2 inoculated dogs were euthanized on day 10 p.i. Sera derived from these animals were negative in the ELISA and HI tests.
The abdomens of four naïve mice were shaved and four injection sites on each mouse were marked (three repeated experimental sites and one negative control site).
Experimental treatment groups included one negative control group (no process) and four heat and microbial treated groups.
In contrast, Alvarez-Alfageme et al. [8] used only one negative control treatment for both toxin treatments.
The TaqMan method was desired two positive control (Allele1 and 2) and one negative control to analyze each SNP locus.
Three stable and one negative control cell lines were harvested by trypsinization and washed three times with cold serum-free medium.
In each independent muti-targeted PCR assay, test results were compared with the results for one positive and one negative control.
Thus, a total of 37 reactions were studied per reference gene per tissue, including one negative control (template control without cDNA).
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