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One ml of medium was placed on top of the solidified cell layer.
One ml of cells was overlaid on pre-soaked filter papers in Petri plates.
One ml of such M. tuberculosis cell suspension was carefully layered on top of the gradient.
One ml of freshly eluted 99mTcO4- (200- 400MBq) was added to the above mixture.
One mL of each supernatant was then transferred into a 1.5 mL Eppendorf tube.
One ml of sample was mixed with 25 μl of 3 M HCl.
One ml of the recovery fluid was also placed directly in a 100 mm Petri plate.
One mL of 1 × 10-3 M TCNQ solution was added and mixed well.
The ethanol extracts were combined, concentrated to dryness and solubilized with one mL of distilled water.
One mL of 40%% Rochelle salt solution was added so as to maintain the color.
One ml of the broth containing the clone was added to 25 ml of PCR reaction mixture.
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