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In particular, one Lb.
One LB variant with an allele frequency of 0.005 in the Dutch population occurred in three patients.
The effect was relatively constant across the different MSLL libraries, with nine of the ten libraries having pairing fractions between 45% and 63%, and only one (Lb) being substantially different (23%).
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One LB-N plate containing 90 mM arbutin was used to plate independently each of the 20 pools and incubated at 37°C.
One LB-N plate containing 90 mM arbutin was used to plate independently 200 CFU of each of the 30 cultures and incubated at 37°C.
The media were also diluted to one-half LB, one-third LB, and one-half 2216, to determine the effect of nutrient concentration on the biomass of the microorganism.
The cells were incubated for one hour before plating on LB or BHI agar plates with 20 μg/mL kanamycin or 4 μg/mL erythromycin.
The mixtures were serially diluted and plated on LB + antibiotic plates (one gentamicin or streptomycin and one tetracycline).
In contrast, three hypothetical genes are active in LB at 15°C, three in LB at pH4, two on solid LB, one in LB with nitrite, and none on LB at pH9 (Table 2, graphic version in Additional file 4).
All analyses were made by one observer (LB).
One observer (LB) carried out a second set of scores after 72 hours.
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CEO of Professional Science Editing for Scientists @ prosciediting.com