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One internal solution for coping with an overwhelming traumatic event is to deny its existence; i.e., to dissociate from the painful truth and proceed as though it didn't happen.
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Summarizing the above information, we conclude that, for every time interval [ t −, t + ], we have at least two solutions which are homoclinic to P + (one external and one internal) and one internal heteroclinic solution from P + to P −.
Recordings were made using 3 5 MΩ fire-polished glass microelectrodes filled with one of two internal solutions: a K-gluconate-based solution for current-clamp experiments and a CsCl-based solution for voltage-clamp recordings.
Labelled serum samples of one patient, one control and one internal standard were pooled together and rehydrating stock solution (8M urea, 2M thiourea, 2% CHAPS, 0.002% bromophenol blue) was added to make up the final volume to 250 μl.
In these experiments we found two populations of neurons distributed around EGABA values, one similar to the ECl− predicted from the internal solution composition and the other depolarized about 30 mV (~0 mV and ~−30 mV) from the value expected from the internal solution (−30 mV and −60 mV).
The internal solution contained CsMeSO3 (see Methods).
Internal solution compositions are described in Patch-clamp recordings below.
The voltages were corrected for the liquid junction potential between external solution and internal solution.
Two types of pipette internal solution were used.
Enrofloxacin: Neoflox® 10% internal solution; Tolnagro Ltd., Szekszárd, Hungary.
To overcome the aforementioned difficulties in PVC membrane electrodes, new kinds of all solid- state ones (without internal reference solution) allowing electrode miniaturization capability were introduced.
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