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Although a significantly higher number of blastocysts were implanted in the MC-reconstituted uterine horn (Table 2), all females had at least one implantation on the mock-treated site (Supplementary Figures 3 and 5).
On day 10 of pregnancy, one implantation site per female was collected for cryosections and one implantation site was collected for paraffin studies with previous fixation in 4% (w/v) PFA with 0.1 M sucrose (pH 7.4) for 6 h.
Doreen's sonogram reveals one implantation.
Most patients (19/22) underwent one implantation session (median number of 10 iodine-125 seeds/session).
A cell suspension containing 1 × 10 cells per 5 μl PBS was used for one implantation into the striatal region of the athymic mouse brain.
Furthermore, using the implantation needle as a bioreactor provides an "all in one" implantation device, easily adapted for standard cell culture equipment (i.e., petri dish, with media, and an incubator).
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Thirteen out of the 112 patients with baseline data and 2-year follow-up data had undergone some sort of further surgery on the same knee, between 1 month and 21 months after the index surgery (eight early wound revisions, including evacuation of haematomas; four revisions with exchange of the implant; and one secondary implantation of a patella component).
In such cases, a couple may choose in vitro fertilization, with genetic analysis of the resulting embryos performed to select a chromosomally normal one for implantation.
However, this difference was not observed in patients who presented with only one previous implantation failure.
However, nitrided samples using the HIPIN technique out perform the one using implantation.
This led to the translation of this scaffold as a one-step implantation without any cell augmentation into the clinical setting.
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