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PCR primers for all eight target genes used for validation and one housekeeping gene Cyclophilin A (CypA) were designed based on 100% homology to the RefSeq as well as mismatch to other genes.
Shortly, this method requires the assignment of one housekeeping gene (β-actin in this case), which is assumed to be constantly expressed in all samples.
The oligonucleotide probes were designed to target eight immune-related genes that involve in antimicrobial activity, melanization, pathogen pattern recognition proteins, lysozyme and one housekeeping gene as an internal control.
We also added one housekeeping gene (GAPDH) to check its suitability for normalisation purposes.
The expression of 23 target genes and one housekeeping gene (GAPDH) was measured with qPCR.
Figure 1A provides the schematic representation of the membrane array with 16 target genes, one housekeeping gene (β-actin), one bacterial gene (TB), and the blank control.
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3. Save on housekeeping.
BETH GIVENS admits she is guilty of avoiding at least one small housekeeping matter.
But turns out zapping your dish sponge for 60 seconds -- with vinegar and lemon juice, if you desire -- actually kills bacteria (up to 99.9percentt, in one Good Housekeeping test).
To obtain a solid basis for normalization of our gene expression data when studying the flower development in cotton, we evaluated the expression stability of nine candidate reference genes, including one traditional "housekeeping" gene in five different experimental sets.
On one occasion, my father's calls to housekeeping went unanswered for hours.
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