Sentence examples for one further hour from inspiring English sources
Afterwards, the cells were washed thrice with PBS and incubated for one further hour with Alexa488 or Alexa633 labeled secondary antibodies (Invitrogen) diluted 1 300 in blocking buffer.
Afterwards, the cells were washed thrice with PBS and incubated for one further hour with an Alexa488 labeled secondary antibody (Invitrogen) diluted 1 300 in blocking buffer.
Fifty μg/mL gentamicin (Sigma) was resuspended in antibiotic-free DMEM, applied to the monolayer, and incubated for one further hour to kill extracellular bacteria.
Thereafter cell layers were blocked for 20 min with 10% blocking reagent (Roche) and incubated for one further hour with 0.5 µg/ml anti-5-hmC antibody (Abgent).
One further hour of assessment will be undertaken using a specially designed probe of 292 single sounds, words and phrases using a PowerPoint presentation to establish baseline performance on treated and untreated experimental stimuli.
After a blocking step with 1% blocking solution (Roche) in TBS for one hour, a solution of 50 ng/µl streptavidin-horse radish peroxidase conjugate (Promega) in TBS was added and incubated for one further hour at room temperature.
One event that seems unlikely now is a further Hour record attempt, building on Wiggins's 54.526km from June.
He had three ejaculations, each one further apart from the last, the final one coming after over an hour of sex.
