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MS spectra were recorded consecutively in one segment with one full scan event in the range of m/z 100.00 650.00.
One full scan (m/z: 450 1600) was followed by maximal 4 MS/MS scans.
Each cycle of one full scan mass spectrum (m/z 400 2000) was followed by four data dependent tandem mass spectra with the collision energy set at 35%.
Although differential pulse voltammetry is more sensitive than the previous techniques [ 133– 135], it has limited time resolution, because one full scan can take longer than seconds.
Continuous cycles of one full scan (m/z 500 to 1700) followed by three data-dependent MS/MS measurements at 35% normalized collision energy were done.
Each cycle of one full scan mass spectrum (m/z 150 2000) was followed by three data-dependent tandem mass spectra on the three most intense peaks.
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Each cycle of one full-scan mass spectrum (m/ z 400 2000) was followed by four data-dependent tandem mass spectra with collision energy set at 35%.
A cycle of one full-scan mass spectrum (400 1400 m/z) followed by 5 data dependent MS/MS scan at a 35% normalized collision energy was repeated continuously throughout each step of the multidimensional separation.
For MS/MS analysis, each scan cycle consisted of one full-scan mass spectrum (with m/z ranging from 400 to 1800 and charge states from 2 to 5) followed by five MS/MS events.
A data-dependent scan composed of one full MS scan (resolution of 60,000) and three CID MS/MS scans (resolution of 7,500) was used as the mass spectrometry method.
Tandem MS were acquired using a data dependent scanning mode, in which one full MS scan (m/z 400 2000) was followed by five MS/MS scans.
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