We designed primers flanking each side of one intron for about 80 genes with Primer3 (Rozen and Skaletsky, 2000), setting the optimal annealing temperature to 60°C (minimum 59°C, maximum 61°C) and Max Poly X to 3, and using default settings for other parameters.
The protein-binding DNA fragment is defined as the longest DNA subsequence bounded by two bases that are in contact with the protein plus one flanking base on each side (5' and 3').
It's flanked on each side by high mountains.
Differently from the notochord of a Ciona tailbud, which consists of 40 cells and is flanked on each side by 18 muscle cells [ 25], the notochord of a tailbud Oikopleura embryo contains only 20 cells (19 columnar cells plus one round terminal cell), flanked on each side by 10 muscle cells [ 9].
Expected heterozygosity [ 5] was calculated for each marker interval on the genome in the simulated population, using one flanking marker on either side of the interval.
In 2012, in a much smaller evaluation, two accessions from wild V. vinifera subsp. sylvestris were identified as resistant from field evaluations: O34-16, collected from Shiravan, Iran, had all four resistance-linked alleles; and DVIT3351.27, collected from Armenia, had the resistance allele at one of the flanking markers on each side.
He did not mean the demographic particulars — a smattering of arty establishments among the bodegas, chop shops and dollar discount stores, with blue-collar Latino blocks flanking one side of the avenue and Hasidic enclaves on the other — so much as the hectic, patchwork ambience of work and idleness, affluence and hardship.
Two genes are found next to Pdu-Gsx, and two genes on the other side next to Pdu-Xlox, and four genes flanking one side of Pdu-Cdx.
He surveyed the hillside, admired the lantana growing from Mexican pottery urns flanking one side of the pool.
Thus the class of unilateral repeated microsatellites similar to LCSC1 has an RTE element flanking one side.
