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These varied observations are not surprising since the LC/MS data are reflective of the more in vivo situation, whereas the incision assay is semi- in vivo that utilizes an oligonucleotide with only one lesion at a defined position.
Interestingly, the dimethylation of only one arginine at a defined position (amino acid 112) was able to increase further the sensitivity of this particular peptide without loss of specificity.
Thus, by introducing only one sDMA and at a defined position (amino acid 112) in SmD3, the sensitivity of binding to this peptide (AARG sDMA GRGMGRGNIF; SMP) was remarkably increased without loss in specificity.
It's a nebulously defined position, yet a critical one.
The SLAM approach presented here is feature-based, thus the map is represented by a set of 3D landmarks each one defined by a global position in space and a visual descriptor.
We determined the peptide-binding specificities of this HLA-DQ molecule by screening a synthetic acetylated nonapeptide amide library with one defined and eight random sequence positions.
The maximum number of defined positions.
No one defined it better.
No one defined him.
When one defines a pattern ocurrence as a position where the PSSM score is above a given threshold, it is possible to associate a regular expression to this particular pattern.
How would one define "settled"?
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com