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Bacterial stocks were stored at -80°C in appropriate media supplemented with 33%% (v/v) glycerol and subcultured three times at one day intervals on fresh agar plates before use.
The sample was withdrawn at regular one day intervals up to 7 days and replaced with the same volume of freshly prepared PBS pH 7.4.
Cell free supernatants were collected at one day intervals by centrifuging culture broths at 10,000 rpm for 30 min at 4°C and used for detection of lipopeptide antimicrobial activity using Kirby-Bauer disk diffusion susceptibility test (Bauer et al. [1966]).
Using a standardised PW Doppler echocardiographic protocol, 10 healthy adult unsedated female Saanen goats were investigated three times at one day intervals by the same observer.
When the tumour length exceeded 0.5 cm, the mice were randomly divided into five groups (10 mice per group) and subsequently injected intraperitoneally with 6 pmol (L), 60 pmol (M), 600 pmol (H) TGFαL3SEAD227A, 60 pmol rSEA or PBS four times at one day intervals.
Forecasts for tropical cyclone movement were extended to two days in advance (at one day intervals) in 1961.
Similar(52)
Prospective DSE-CE and DSE-TEE with maximum one day interval were performed in a random order.
Floret organs infected with TRI5prom:: GFP strain from attached in vivo assays were examined at a one day interval from 3 dpi to 7 dpi.
Frozen aliquots were either kept at −80°C or subjected to 5 thawing/freezing cycles at minimum one day interval before use.
In general, consecutive flower opening occurred at one-day intervals 75% of the time, until the 16th flower on a given inflorescence (Fig. 1C).
He teaches six days a week so we had to film his part in one-day intervals.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com