All subjects participated in one experimental day approximately 3 weeks before 10 days of strict bed rest (BR).
The budget for measuring one cluster was fixed to one day work, approximately 420 min.
So we had to cut our holiday short by one day, thereby losing approximately £120 and having to leave a fridge full of food.
For cryopreserving, donor leukocytes were resuspended in a cryopreserving media consisting of 90% FBS +10% Dimethyl Sulfoxide (DMSO) and stored first in a -80°C freezer for approximately one day before being transferred to liquid nitrogen for approximately one month.
In small facilities, data collection took approximately one day, but larger facilities required several days.
For example, after treatment with 0.5 mM H2O2 approximately 30% of wild type larvae were permanently arrested at the L2/L3 stage, 20% were unaffected and approximately 50% were temporarily delayed in development because they reached adulthood approximately one day later than did untreated controls.
One day prior to transfection, approximately 60,000 to 70,000 Cos7 and Hek293 cells were seeded and transfections carried out using approximately 0.4 μg of recombinant construct (pcDNA3+sbPAC1A or B) and cells were grown for 2 days at 37°C.
In a kinetic study, dividing T cells were found to express high levels of Bcl6 2 days after immunization, while they did not express CXCR5 until approximately one day later (Baumjohann et al., 2011).
The term "near-real-time analysis" used throughout this article can be defined as the process of collecting the information available within the first 0 5 days of a disaster, analyzing that information, and producing results within approximately one day of the latest information.
The half-life for oxidation of free sulfide in the presence of dissolved oxygen is approximately one day [27, 28].
