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One brood each year is normal.
This species is generally monogamous, and produces one brood each year.
The eggs are incubated by the female alone, though the male brings her food as she nests, and most mating pairs raise only one brood each year.
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One brood in each cage came from each of the two treatments.
This study focused on one brood group of 25 males and 25 females designated 'B2' (Table 1).
To do so, we performed a brood size manipulation experiment in the study population: broods hatched on the same day were matched in triplets, and part of the hatchlings were exchanged among broods of each triplet to create one brood reduced by two nestlings, one control brood (i.e. non-manipulated number of nestlings) and one brood enlarged by two nestlings within each triplet.
In one brood (c22), two larvae (0.48% of all genotyped larvae) were each homozygous at a single microsatellite locus for an allele that differed from the mother's, violating assumptions of Mendelian segregation.
Multivoltine insects lay more than one brood per year, and their classification as semelparous or iteroparous may depend on the time scale of reference [ 18].
Comparisons between treatments (untreated, sterile injury and septic injury) were performed within one brood.
In one of the two experiments that failed, only one brood (84 F1's) was screened, which may have been too few (experiment 21).
We hypothesize that this may be due to higher breeding stress and reduced fitness of the parents after having already fledged one brood.
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